Deivanayaki M, Antony Iruthayaraj P
Nehru Arts and Science College, Coimbatore – 641 105, Tamilnadu, India
Department of Biotechnology, Bharathiar University, Coimbatore – 641 046, Tamilnadu, India
Key words: Culture media, media from local raw materials, conventional media, media formulation.
The high cost of the microbial culture media paved a way for the production of alternative media using cheap local raw materials (Potato, Groundnut, Cereals, Cassava, Yam, Pigeon pea, Maize and Beans). The vegetables being a good source of nutrients are used to formulate both solid and liquid media for the growth of selected bacteria and fungi. Their growth on the formulated media was compared with growth on the conventional media. All the formulations produced good growth of microbes similar to the conventional media.
In the solid media formulation, Staphylococcus sp., produced 230 CFU / ml in Formulation E, 150 CFU / ml of Escherichia coli in Formulation A, 170 CFU / ml of Salmonella sp., in Formulation B, 150 CFU / ml of Klebsiella sp., in Formulation B, 180 cells / 0.1 ml of Aspergillus sp., in Formulation A and 150 cells / 0.1 ml of yeast in Formulation C. In broth formulations, Staphylococcus sp., Escherichia coli, Klebsiella sp., and Salmonella sp., produced 250, 200, 150, 175 cells / 0.1 ml of sample in the Formulations I, G, H and J respectively. Comparing with the performance on conventional bacteriological and mycological media, the prepared vegetable media is found to be a good and cheap media material for the isolation and cultivation of both bacteria and fungi.
Get the original articles in Source: Volume 2, Number 5, May 2012 – IJB
Published By: International Journal of Biosciences (IJB)